NS5-V372A and NS5-H386Y variations are responsible for differences in interferon α/β induction and co-contribute to the replication advantage of Japanese encephalitis virus genotype I over genotype III in ducklings
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The siRNA-269 (5′-UCCAACACCUCUUCGACACTT-3′) and siRNA-425 (5′-GCAACCUUCACCUCAGCAUTT-3′) targeting duck IFN-α mRNA (GenBank no. AB128861.1), and siRNA-272 (5′-UCCAACACCUCUUCAACAUTT3′) and siRNA-464 (5′-GCAUCCAACACUUCCUCCATT-3′) targeting duck IFN-β mRNA (GenBank no. KM035791.2) were designed using GenScript (https://www.genscript.com/ssl-bin/app/rnai) and RNA wizard (http://www.sirnawizard.com/design_advanced.php), and blasted against the reference sequence database of duck to red.... |
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