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PCR Cloning and Subcloning> | … The plasmid psCMVcd::uprt containing the yeast cytosine deaminase::phosphoribosyl transferase fusion protein [7] was built by cloning the corresponding gene sequence (optimized for transcription in mammalian cells and synthesized by GenScript, Piscataway, NJ) between … | Get A Quote |
We tested the efficacy of a yeast cytosine deaminase::uracil phosphoribosyl transferase/5-fluorocytosine (CDU/5-FC) non-viral suicide system on eight established canine melanoma cell lines. Albeit with different degree of sensitivity five days after lipofection, this system was significantly efficient killing melanoma cells, being four cell lines highly, two fairly and two not very sensitive to CDU/5-FC (their respective IC ranging from 0.20 to 800 μM 5-FC). Considering the relatively low lipofection efficiencies, a very strong bystander effect was verified in the eight cell lines: depending on the cell line, this effect accounted for most of the induced cell death (from 70 to 95 %). In our assay conditions, w... More