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ORF cDNA Clones/MolecularCloud> | … AT-Hook 3 (KRPRGRPRKW, 1338 Da) were obtained from GenScript (Piscataway, NJ). All peptide solutions were analyzed at a concentration of 5 μM in 50:50 water/methanol (H2O/MeOH) solvent conditions with 0.1% formic acid (FA). A low concentration Tuning Mix standard (… | Get A Quote |
In the present work, four, well-studied, model peptides (, substance P, bradykinin, angiotensin I and AT-Hook 3) were used to correlate structural information provided by ion mobility and ECD/CID fragmentation in a TIMS-q-EMS-ToF MS/MS platform, incorporporating an electromagnetostatic cell (EMS). The structural heterogeneity of the model peptides was observed by (i) multi-component ion mobility profiles (high ion mobility resolving power, ∼115-145), and (ii) fast online characteristic ECD fragmentation patterns per ion mobility band (∼0.2 min). Particularly, it was demonstrated that all investigated species were probably conformers, involving /-isomerizations at X-Pro peptide bond, following the same prot... More