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GenParts™ DNA Fragments> | … Antibodies were purified from supernatants using AmMag Protein A Magnetic Beads (Genscript) according to the manufacturer’s protocol. … To remove Fc-fragments and non-digested IgG the reaction was subsequently incubated with AmMag Protein A magnetic beads (Genscript… | Get A Quote |
The V3 loop of the HIV-1 envelope (Env) protein elicits a vigorous, but largely non-neutralizing antibody response directed to the V3-crown, whereas rare broadly neutralizing antibodies (bnAbs) target the V3-base. Challenging this view, we present V3-crown directed broadly neutralizing Designed Ankyrin Repeat Proteins (bnDs) matching the breadth of V3-base bnAbs. While most bnAbs target prefusion Env, V3-crown bnDs bind open Env conformations triggered by CD4 engagement. BnDs achieve breadth by focusing on highly conserved residues that are accessible in two distinct V3 conformations, one of which resembles CCR5-bound V3. We further show that these V3-crown conformations can, in principle, be attacked by antibo... More