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GenParts™ DNA Fragments> | … The plasmid constructs were constructed either by NEBuilder HiFi DNA Assembly Master Mix (NEB) to clone DNA fragments (from Integrated DNA Technologies) into the pET11a vector, or by DNA synthesis using Genscript. The plasmids were transformed into E.coli strain BL21(… | Get A Quote |
Current fire retardants are known to be toxic to humans and our environment. As environmental-friendly flame retardants (FRs), protein-based flame retardants have been studied extensively recently, even though they are not durable. In this study, we designed, synthesized and tested a durable protein-based FR through the fusion of the adhesion domain from either mussel foot protein-5 (mfp-5) or cellulose-binding domain (CBD) with flame retardant protein (SR protein and alpha casein). We first verified the expression of the recombinant proteins in using Western blot. Then, we coated the fusion protein (carrying cell lysates) to cotton fabrics and wood and verified with Infrared (IR) spectroscopy. Using a vertica... More