Products/Services Used | Details | Operation |
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GenParts™ DNA Fragments> | … The synthetic gene fragments were obtained from GenScript and IDT. Lentiviruses were produced using HEK293T cells, following a standard calcium precipitation protocol and an ultracentrifigation concentration protocol as previously described 27 , 28 . Lentivector titers were … | Get A Quote |
Cell-based immunotherapy has become the new-generation cancer medicine, and "off-the-shelf" cell products that can be manufactured at large scale and distributed readily to treat patients are necessary. Invariant natural killer T (iNKT) cells are ideal cell carriers for developing allogeneic cell therapy because they are powerful immune cells targeting cancers without graft-versus-host disease (GvHD) risk. However, healthy donor blood contains extremely low numbers of endogenous iNKT cells. Here, by combining hematopoietic stem cell (HSC) gene engineering and differentiation, we generate human allogeneic HSC-engineered iNKT (HSC-iNKT) cells at high yield and purity; these cells closely resemble endogenous iNKT... More