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G protein-coupled receptor kinase-2 confers isoform-specific calcium sensitivity to dopamine D receptor desensitization

FASEB J. 2021-11; 
Richard Ågren, Kristoffer Sahlholm
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DNA Sequencing … ), and melanin-concentrating hormone 2 receptor (MCH 2 R; GenScript, Piscataway, NJ) cDNA were in pXOOM (provided by Dr Søren-Peter … 195 C-terminal residues of GRK2 14 were prepared by Genscript and the resulting constructs were sequenced in their entirety. Plasmids … Get A Quote

摘要

The dopamine D receptor (D R) functions as an autoreceptor on dopaminergic cell bodies and terminals and as a postsynaptic receptor on a variety of neurons in the central nervous system. As a result of alternative splicing, the D R is expressed as two isoforms: long (D R) and short (D R) differing by a stretch of 29 residues in the third intracellular loop, with D R being the predominant presynaptic isoform. Recent reports described a Ca sensitivity of the desensitization time course of potassium currents elicited via D R, but not via D R, when either isoform was selectively expressed in dopaminergic neurons. Here, we aimed to study the mechanism behind this subtype-specific Ca sensitivity. Thus, we measured th... More

关键词

Xenopus oocytes, beta-arrestin, dopamine receptor isoforms, kinase inhibitors, voltage clamp