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Custom Vector Construction> | … According to the genomic sequence of ASFV HLJ/18, the cDNAs corresponding to 94 ASFV-encoded proteins were synthesized by GenScript incorporated (Nanjing, China) and cloned into the pCAGGS-Flag vector. The cDNAs corresponding to BCL-2 family members (BAK, … | Get A Quote |
African swine fever (ASF) is a severe hemorrhagic disease in swine characterized by massive lymphocyte depletion and cell death, with apoptosis and necrosis in infected lymphoid tissues. However, the molecular mechanism regarding ASFV-induced cell death remains largely unknown. In this study, 94 ASFV-encoded proteins were screened to determine the viral proteins involved in cell death in vitro, and pE199L showed the most significant effect. Ectopic expression of pE199L in porcine cells (CRL-2843) and human cells (HEK293T and HeLa cells) induced cell death remarkably, showing obvious shrinking, blistering, apoptotic bodies, and nuclear DNA fragments. Meanwhile, cell death was markedly alleviated when the express... More