| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | … After finding the optimal expression vector, the Nmar 1308 gene construct was purchased from Genscript Biotech (codon-optimized with cleavable N-terminal hexa-Histidine tag). Within the gene, NdeI and BamHI endonuclease restriction sites were used to insert Nmar_1308 … | Get A Quote |
The ammonia-oxidizing thaumarchaeal 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) cycle is one of the most energy-efficient CO fixation cycles discovered thus far. The protein encoded by Nmar_1308 (from Nitrosopumilus maritimus SCM1) is a promiscuous enzyme that catalyzes two essential reactions within the thaumarchaeal 3HP/4HB cycle, functioning as both a crotonyl-CoA hydratase (CCAH) and 3-hydroxypropionyl-CoA dehydratase (3HPD). In performing both hydratase and dehydratase activities, Nmar_1308 reduces the total number of enzymes necessary for CO fixation in Thaumarchaeota, reducing the overall cost for biosynthesis. Here, we present the first high-resolution crystal structure of this bifunctional enzyme w... More
