Products/Services Used | Details | Operation |
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Custom DNA/RNA Oligos> | … PCR reaction contains 2 μL of genomic DNA, 10 μL Premix Taq (Takara Biomedical Technology), 2 μL of each primer (10 μM, synthesized by Genscript China Inc.), and adds distilled-deionized water to 20 μL. PCR program was 1 cycle of 95 C for 5 min, 35 cycles of 95 C for 30 s… | Get A Quote |
The secretions of the venom glands, dry skin and whole body of several Bufo species have been used as traditional medicines in East Asia to treat heart failure and cancer. Due to the highly similar morphological features of Bufo species and their derived commercial crude drugs, along with the high similarity of chemical composition of the secretions of venom glands, it is very challenging to identify the medicinal toads and the related crude drugs. The cyt-b sequences provide useful information to authenticate medicinal Bufo species. Based on the cyt-b sequences, a simple PCR-RFLP method was established for the identification of the medicinally used Bufo species as well as their derived crude drugs. The 23 spec... More