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Custom DNA/RNA Oligos> | … pulse off in ice-cold bathroom). The sonicated mixture was centrifuged at 10,000g for 20 min at 4°C, and the supernatant containing a soluble fraction was loaded onto High-Affinity Ni-Charged Resin (GenScript, USA). After being … | Get A Quote |
In our previous study, we reported the design and recombinant production of the p28-apoptin as a novel chimeric protein for breast cancer (BC) treatment. This study aimed to evaluate the inhibitory activity of the chimeric protein against BC cells in vitro and in vivo. We developed a novel multifunctional protein, consisting of p28, as a tumor-homing killer peptide fused to apoptin as a tumor-selective killer. The chimeric protein showed significantly higher toxicity in BC cell lines dose-dependently than in non-cancerous control cell lines. IC values were 1.41, 1.38, 6.13, and 264.49 μM for 4T1, MDA-MB-468, Vero, and HEK293 cells, respectively. The protein showed significantly enhanced uptake in 4T1 cancer ... More