Products/Services Used | Details | Operation |
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Recombinant Antibody Expression> | … To detect CAR expression, CAR transduced cells were stained with biotinylated recombinant Protein L (GenScript), followed by APC-conjugated streptavidin (BD Biosciences). PE conjugated HLA-A*2402/LMP2131-139 tetramer, A*2402/LMP2419-427 tetramer, or A*2402/… | Get A Quote |
We generated dual-antigen receptor (DR) T cells from induced pluripotent stem cells (iPSCs) to mitigate tumor antigen escape. These cells were engineered to express a chimeric antigen receptor (CAR) for the antigen cell surface latent membrane protein 1 (LMP1; LMP1-CAR) and a T cell receptor directed to cell surface latent membrane protein 2 (LMP2), in association with human leucocyte antigen A24, to treat therapy-refractory Epstein-Barr virus-associated lymphomas. We introduced LMP1-CAR into iPSCs derived from LMP2-specific cytotoxic T lymphocytes (CTLs) to generate rejuvenated CTLs (rejTs) active against LMP1 and LMP2, or DRrejTs. All DRrejT-treated mice survived >100 days. Furthermore, DRrejTs rejected fo... More