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Plasmid DNA Preparation> | … Similarly, cDNAs of Seg-2 (VP2) and Seg-6 (VP5) of different serotypes in an appropriate plasmid for run-off RNA synthesis were synthesized by Genscript Corporation Piscataway, NJ, USA (Table 1). In general, these cDNAs consist of open reading frames of the respective … | Get A Quote |
Bluetongue (BT) is a midge-borne OIE-notifiable disease of ruminants caused by the bluetongue virus (BTV). There are at least 29 BTV serotypes as determined by serum neutralization tests and genetic analyses of genome segment 2 encoding serotype immunodominant VP2 protein. Large parts of the world are endemic for multiple serotypes. The most effective control measure of BT is vaccination. Conventionally live-attenuated and inactivated BT vaccines are available but have their specific pros and cons and are not DIVA compatible. The prototype Disabled Infectious Single Animal (DISA)/DIVA vaccine based on knockout of NS3/NS3a protein of live-attenuated BTV, shortly named DISA8, fulfills all criteria for modern vete... More