Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning> | … The construct rhb1 swap, having the introns of rhb1 in reverse order, was synthesized (GenScript) and PCR amplified with the primers described above. Deletion fragments of rhb1 were derived from the full length constructs and where constructed with the same cloning strategy … | Get A Quote |
Splicing of mRNA precursors is essential in the regulation of gene expression. U2AF65 recognizes the poly-pyrimidine tract and helps in the recognition of the branch point. Inactivation of fission yeast U2AF65 (Prp2) blocks splicing of most, but not all, pre-mRNAs, for reasons that are not understood. Here, we have determined genome-wide the splicing efficiency of fission yeast cells as they progress into synchronous meiosis in the presence or absence of functional Prp2. Our data indicate that in addition to the splicing elements at the 3' end of any intron, the nucleotides immediately upstream the intron will determine whether Prp2 is required or dispensable for splicing. By changing those nucleotides in any g... More