Products/Services Used | Details | Operation |
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Monoclonal Antibody Services> | … Customized rabbit anti-PbrDGK4 monoclonal antibody (1: 500; GenScript, Nanjing, China) was used as the primary antibody. The DGK4 antibody was prepared against a specific DGK4 peptide (Figure S3). The specificity of the antibody was determined by immunoprecipitation (… | Get A Quote |
Pear has an S-RNase-based gametophytic self-incompatibility (SI) system. Nuclear DNA degradation is a typical feature of incompatible pollen tube death, and is among the many physiological functions of vacuoles. However, the specific changes that occur in vacuoles, as well as the associated regulatory mechanism in pear SI, are currently unclear. Although research in tobacco has shown that decreased activity of diacylglycerol kinase (DGK) results in the morphological change of pollen tube vacuole, whether DGK regulates the pollen tube vacuole of tree plants and whether it occurs in SI response, is currently unclear. We found that DGK activity is essential for pear pollen tube growth, and DGK4 regulates pollen tu... More