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Mammalian Expression> | … The 293T and human hepatocyte line L02 (Shanghai Institute of Cellular Biology of Chinese Academy of Sciences, Shanghai, China) were … antagomiR- 494 and antagomiR- NC were purchased from GenScript (Nanjing, Jiangsu, China). The rats were administered via tail vein 3 … | Get A Quote |
Hepatic ischaemia-reperfusion (I/R) injury constitutes a tough difficulty in liver surgery. Dexmedetomidine (Dex) plays a protective role in I/R injury. This study investigated protective mechanism of Dex in hepatic I/R injury. The human hepatocyte line L02 received hypoxia/reoxygenation (H/R) treatment to stimulate cell model of hepatic I/R. The levels of pyroptosis proteins and inflammatory factors were detected. Functional rescue experiments were performed to confirm the effects of miR-494 and JUND on hepatic I/R injury. The levels of JUND, PI3K/p-PI3K, AKT/p-AKT, Nrf2, and NLRP3 activation were detected. The rat model of hepatic I/R injury was established to confirm the effect of Dex in vivo. Dex reduced py... More