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Mammalian Expression> | … Based on the identification of fucosylation sites in mammalian cells and in planta assays, CPN20 peptide was synthesized (GenScript) for the in vitro enzyme assays, KIDITVPTGAQIIYSK (amino acid residues 112-127). Each 20 μl of reaction included 150 μM CPN20 peptide, … | Get A Quote |
is involved in some aspects of plant development. However, the nature of this protein as an -fucosyltransferase was recently discovered. In this study, we show that (SPY) interacts with CPN20 in yeast two-hybrid and split-luc assays, and the interaction is promoted by ABA. CPN20 is a chloroplast-localized co-chaperonin that negatively regulates ABAR-mediated ABA signaling. By using Electron Transfer Dissociation-MS/MS analysis, two -fucosylation sites, e.g., 116th and 119th threonines, were detected in ectopically expressed CPN20 in mammalian cells and in . The -fucosylation at both threonine residues was confirmed by peptide -fucosylation assay. We further show that CPN20 accumulates in the chloroplast of m... More