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Mammalian Expression> | … Recombined heavy chain (VDJ) and light chain (VJ) Ig sequences were synthesised (Genscript, Piscataway) and cloned into expression plasmids encoding the constant regions of human IgG1. The expression plasmids were then transfected into Expi293F cells using … | Get A Quote |
Humans are exposed to influenza virus through periodic infections. Due to these repeated exposures, human populations commonly have elevated antibody titers targeting the conserved internal influenza virus nucleoprotein (NP). Despite the presence of anti-NP antibodies, humans are acutely susceptible to drifted influenza viruses with antigenically different surface proteins and the protective potential of human NP antibodies is unclear. In this study, high levels of anti-NP antibody and NP-specific B cells were detected in both adult humans and influenza-infected mice, confirming that NP is a major target of humoral immunity. Through sorting single B cells from influenza-exposed human adults, we generated a pane... More