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GenParts™ DNA Fragments> | … Plasmid insertion fragments for reporter assay and real-time PCR were synthesized by GenScript (Nanjing, China). Yeast extract, glucose, leucine, tryptone, agar and thiamine for strain culture were purchased from Sigma. Phenol (pH 4.3 ± 0.2) and EDTA for RNA extraction … | Get A Quote |
The twister ribozyme is widely distributed over numerous organisms and is especially abundant in Schistosoma mansoni, but has no confirmed biological function. Of the 17 non-LTR retrotransposons known in S. mansoni, none have thus far been associated with ribozymes. Here we report the identification of novel twister variant (T-variant) ribozymes and their function in S. mansoni non-LTR retrotransposition. We show that T-variant ribozymes are located at the 5' end of Perere-3 non-LTR retrotransposons in the S. mansoni genome. T-variant ribozymes were demonstrated to be catalytically active in vitro. In reporter constructs, T-variants were shown to cleave in vivo, and cleavage of T-variants was sufficien... More