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GenParts™ DNA Fragments> | … A; Additional file 1) were cloned into pUC57 (GenScript) and fragments were joined sequentially into a single plasmid (pAS2) encompassing the complete sequence of ASncmtRNA-2. The correct sequence and orientation of the 3 fragments in the complete clone was … | Get A Quote |
background: The antisense noncoding mitochondrial RNAs (ASncmtRNAs) derive from the mitochondrial 16S gene. Knockdown of these transcripts with chemically-modified antisense oligonucleotides induces proliferative arrest, apoptosis and invasiveness reduction in tumor but not normal cells. One of these transcripts, ASncmtRNA-2, contains the complete and identical sequence of hsa-miR-4485-3p and, upon knockdown of this transcript, there is a strong increase in levels of this miRNA, suggesting ASncmtRNA-2 as a source for miR-4485-3p, which is supported by several evidences from our group and others, in the ex vivo setting. results: Here we show that incubation of in vitro-transcribed ASncmtRNA-2 with recombinant Di... More