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DNA Sequencing> | … For PAQR4 overexpression, full-length PAQR4 coding sequences were amplified and cloned into pcDNA3.1 expression vector (GenScript, Nanjing, China). PAQR4 overexpression plasmids or vector plasmids were transfected into Huh-7 cells. Lipofectamine 2000 (11668-019; … | Get A Quote |
Progestin and adipoQ receptor 4 (PAQR4) is a novel tumorigenic factor that promotes cell proliferation and metastasis in lung and breast cancer, but its role in hepatocellular carcinoma (HCC) is unknown. The aim of our study was to explore its role and underlying mechanism in the development of HCC. Analysis of GEPIA database indicated that PAQR4 was highly expressed in HCC samples, and the mRNA level of PAQR4 was negatively correlated with the overall survival of HCC patients. Knockdown of PAQR4 in Hep3B cells suppressed cell proliferation by hindering G1/S transition of cell cycle as shown by the flow cytometry analysis. PAQR4 knockdown also expedited the cell apoptosis. Knockdown of PAQR4 repressed the migra... More