Products/Services Used | Details | Operation |
---|---|---|
Custom Vector Construction> | … All the gene constructs were chemically synthesized (GenScript, Nanjing, China) as DNA concatemers and cloned into pET30a(+) vector (Novagen, San Diego, CA, USA) via double enzyme digestion with NdeI and XhoI (New England Biolabs, Ipswich, MA, … | Get A Quote |
African swine fever (ASF) is a highly fatal swine disease threatening the global pig industry. Currently, vaccine is not commercially available for ASF. Hence, it is desirable to develop effective subunit vaccines against ASF. Here, we expressed and purified two recombinant fusion proteins comprising ASFV proteins p30 and p54 fused to a novel cell-penetrating peptide Z12, which were labeled as ZPM (Z12-p30-modified p54) and ZPMT (Z12-p30-modified p54-T cell epitope). Purified recombinant p30 and modified p54 expressed alone or fused served as controls. The transduction capacity of these recombinant proteins was assessed in RAW264.7 cells. Both ZPM and ZPMT exhibited higher transduction efficiency than the other... More