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Custom Vector Construction> | … The full cassette was synthesized by Genscript company (Piscataway, NJ, USA) and cloned into pK7WG2D (Karimi et al. ) using a HindIII site, while the original pRolD-GFP-ter35S was removed to give the pK7WG2D-GFFP vector. In this vector, we also replaced the ccdB with a … | Get A Quote |
Meganucleases are rare cutting enzymes that can generate DNA modifications and are part of the plant genome editing toolkit although they lack versatility. Here, we evaluated the use of two meganucleases, I-SceI and a customized meganuclease, in tomato and oilseed rape. Different strategies were explored for the use of these meganucleases. The activity of a customized and a I-SceI meganucleases was first estimated by the use of a reporter construct GFFP with the target sequences and enabled to demonstrate that both meganucleases can generate double-strand break and HDR mediated recombination in a reporter gene. Interestingly, I-SceI seems to have a higher DSB efficiency than the customized meganuclease: up to 6... More