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Custom Vector Construction> | … The tRNA–gRNA construct was synthesized by GenScript and cloned into a pUC57 vector by GenScript. This vector was then digested with BsaI to extract the tRNA–gRNA construct and ligated to the pRGEB32 vector. The end result was the pRGEB32 binary vector with either … | Get A Quote |
Bottlenecks in plant transformation and regeneration have slowed progress in applying CRISPR/Cas-based genome editing for crop improvement. Rice ( L.) has highly efficient transformation protocols, along with reasonably efficient protocols using immature embryos. However, rapid and efficient protocols are not available for transformation and regeneration in varieties, even though they represent the majority of rice production in the U.S. and South America. The current study has optimized a protocol using callus induction from mature seeds with both -mediated and biolistic transformation of the high-yielding U.S. cultivar Presidio. Gene editing efficiency was tested by evaluating knockout mutations in the (... More