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Custom Vector Construction> | … The cDNAs encoding both human SIX1 SD-HD containing residues 1-189 and its HD (G124-K185) were synthesized by Genscript. The sequences were cloned into pET15b vectors using NdeI and XhoI restriction enzymes. The resulting plasmids were transformed into … | Get A Quote |
Human sine oculis homeobox homolog (SIX) 1 contains a homeodomain (HD), which is important for binding to DNA. In this study, we carried out structural studies on the HD of human SIX1 using nuclear magnetic resonance (NMR) spectroscopy. Its secondary structures and dynamics in solution were explored. HD is well-structured in solution, and our study shows that it contains three α-helices. Dynamics study indicates that the N- and C-terminal residues of HD are flexible in solution. HD of human SIX1 exhibits molecular interactions with a short double-strand DNA sequence evidenced by the H- N-heteronuclear single quantum correlation (HSQC) and F-NMR experiments. Our current study provides structural information f... More