至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Secondary structures, dynamics, and DNA binding of the homeodomain of human SIX1

J Pept Sci. 2021-10; 
Yan Li, Elizabeth YiHui Ng, Ying Ru Loh, Chong Yu Gea, Qiwei Huang, Qingxin Li, CongBao Kang
Products/Services Used Details Operation
Custom Vector Construction … The cDNAs encoding both human SIX1 SD-HD containing residues 1-189 and its HD (G124-K185) were synthesized by Genscript. The sequences were cloned into pET15b vectors using NdeI and XhoI restriction enzymes. The resulting plasmids were transformed into … Get A Quote

摘要

Human sine oculis homeobox homolog (SIX) 1 contains a homeodomain (HD), which is important for binding to DNA. In this study, we carried out structural studies on the HD of human SIX1 using nuclear magnetic resonance (NMR) spectroscopy. Its secondary structures and dynamics in solution were explored. HD is well-structured in solution, and our study shows that it contains three α-helices. Dynamics study indicates that the N- and C-terminal residues of HD are flexible in solution. HD of human SIX1 exhibits molecular interactions with a short double-strand DNA sequence evidenced by the H- N-heteronuclear single quantum correlation (HSQC) and F-NMR experiments. Our current study provides structural information f... More

关键词

DNA binding, NMR, SIX1, protein dynamics, transcription factor