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Custom DNA/RNA Oligos> | … (48,49) Briefly, the ZIKV RdRp coding region was amplified using a synthetic NS5 gene construct as a template (synthesized by GenScript) based upon the NS5 amino acid sequence of the Zika virus strain BeH815744 (AMA12087.1), oligonucleotides 5′-… | Get A Quote |
The naturally occurring nucleotide 3'-deoxy-3',4'-didehydro-cytidine-5'-triphosphate () was recently found to exert potent and broad-spectrum antiviral activity. However, nucleoside 5'-triphosphates in general are not cell-permeable, which precludes the direct use of as a therapeutic. To harness the therapeutic potential of this endogenous antiviral nucleotide, we synthesized phosphoramidate prodrug HLB-0532247 () and found it to result in dramatically elevated levels of in cells. We compared and 3'-deoxy-3',4'-didehydro-cytidine () and found that more effectively reduces titers of Zika and West Nile viruses in cell culture with minimal nonspecific toxicity to host cells. We conclude that is a promising an... More