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Label-Free Colorimetric Method for Detection of by Trimming the G-Quadruplex DNAzyme with CRISPR/Cas12a

Anal Chem. 2021-10; 
Xueyun Chen, Liu Wang, Fang He, Ganghui Chen, Linlin Bai, Kaiyu He, Fang Zhang, Xiahong Xu
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Bacterial Expression … Columns for Ni sepharose were purchased from GenScript (Nanjing, China). Coomassie brilliant blue staining solution and Escherichia coli (E. coli) competent BL21 (DE3) were purchased from Tiangen (Beijing, China). … Get A Quote

摘要

(), which may cause gastrointestinal disorders in humans, is a pathogen commonly found in seafood. There are many methods for detecting , yet they have some shortcomings, such as high cost, labor-intensiveness, and complicated operation, which are impractical for resource-limited settings. Herein, we present a sequence-specific, label-free, and colorimetric method for visual detection of . This method utilizes CRISPR/Cas12a to specifically recognize the loop-mediated isothermal amplification (LAMP) products for further trans-cleaving the G-quadruplex DNAzyme and depriving its peroxidase-mimicking activity. In this way, the results can be directly observed with the naked eyes the color development of 2,2'-azino... More

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