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Recombinant Antibody Expression> | … The recombinant plasmids encoding ESR1, PGR, ERBB2, and GAPDH genes as standards were synthesized by GenScript (Nanjing, China). The sequences of PGR, ESR1, ERBB2 and GAPDH mRNAs were derived from the NCBI reference sequence XM_011542869.2, … | Get A Quote |
Breast cancer has become the leading cause of global cancer incidence and a serious threat to women's health. Accurate diagnosis and early treatment are of great importance to prognosis. Although clinically used diagnostic approaches can be used for cancer screening, accurate diagnosis of breast cancer is still a critical unmet need. Here, we report a 4-plex droplet digital PCR technology for simultaneous detection of four small extracellular vesicle (sEV)-derived mRNAs (PGR, ESR1, ERBB2 and GAPDH) in combination with machine learning (ML) algorithms to improve breast cancer diagnosis. We evaluate the diagnsotic results with and without the assistance of the ML models. The results indicate that ML-assisted anal... More