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Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils In Vitro

Dis Markers. 2021-09; 
Gang Wang, Jun Guan, Guojun Li, Fengtian Wu, Qin Yang, Chunhong Huang, Junwei Shao, Lichen Xu, Zixuan Guo, Qihui Zhou, Haihong Zhu, Zhi Chen
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Proteins, Expression, Isolation and Analysis … Briefly, cells were lysed with SDS sample buffer (1x), boiled for 10min, separated using 4–20% SDS-PAGE (GenScript, USA), and then transferred onto a 0.22μm polyvinylidene difluoride (PVDF) membrane (Millipore, USA). After blocking for 1 h at room temperature with 3% … Get A Quote

摘要

Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently the most significant public health threat worldwide. Patients with severe COVID-19 usually have pneumonia concomitant with local inflammation and sometimes a cytokine storm. Specific components of the SARS-CoV-2 virus trigger lung inflammation, and recruitment of immune cells to the lungs exacerbates this process, although much remains unknown about the pathogenesis of COVID-19. Our study of lung type II pneumocyte cells (A549) demonstrated that ORF7, an open reading frame (ORF) in the genome of SARS-CoV-2, induced the production of CCL2, a chemokine that promotes the chemotaxis of monocyt... More

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