| Products/Services Used | Details | Operation |
|---|---|---|
| ORF cDNA Clones/MolecularCloud | … This distribution of rare codons throughout caf1R ORF was further validated by another rare codon analysis tool (GenScript, Piscataway, NJ, USA). Additionally, the codon usage frequency for the expression of native caf1R in an E. coli host was predicted to be 0.6, about half of … | Get A Quote |
The bacterial pathogen, , has caused three historic pandemics and continues to cause small outbreaks worldwide. During infection, assembles a capsule-like protective coat of thin fibres of Caf1 subunits. This F1 capsular antigen has attracted much attention due to its clinical value in plague diagnostics and anti-plague vaccine development. Expression of F1 is tightly regulated by a transcriptional activator, Caf1R, of the AraC/XylS family, proteins notoriously prone to aggregation. Here, we have optimised the recombinant expression of soluble Caf1R. Expression from the native and synthetic codon-optimised cloned in three different expression plasmids was examined in a library of host strains. The functional... More
