Products/Services Used | Details | Operation |
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Nucleic Acid Purification & Analysis> | … 5.0 primer design software according to the barley-related target gene nucleic acid sequence published on NCBI, and synthesized by GenScript Biotechnology Co., Ltd. (Nanjing, China). Total RNA was extracted from barley seedlings with Plant RNA Extraction Kit (Takara, cat. … | Get A Quote |
It has been revealed that high NaCl stress (>60 mmol L) induced phenolics accumulation in barley seedlings, with γ-aminobutyric acid (GABA) playing a key role. Interestingly, low NaCl stimulus (20 mmol L) enhancing phenolics synthesis and growth of barley seedlings was also reported recently. Hence, exogenous GABA and its bio-synthesis inhibitor 3-mercaptopropionic acid (3-MP) were applied to reveal the mechanism of GABA regulating phenolics metabolism in barley seedlings treated with 20 mmol L NaCl. The contents of total phenolics and flavonoids significantly increased by 11.64% and 14.52% under NaCl, respectively. The addition of GABA further increased phenolics and flavonoids contents, especially for gallic... More