Products/Services Used | Details | Operation |
---|---|---|
Mutant Libraries> | … cereus ATCC 14579, GenScript) were synthesized and cloned into the pET-22b(+) plasmid using restriction enzymes NdeI and HindIII (GenScript), and transformed into competent E. coli BL21 (DE3) cells (Novagen). For the TrxRmutant, mutations were introduced by GenScript … | Get A Quote |
Low-molecular-weight (low M ) thioredoxin reductases (TrxRs) are homodimeric NADPH-dependent dithiol flavoenzymes that reduce thioredoxins (Trxs) or Trx-like proteins involved in the activation networks of enzymes, such as the bacterial class Ib ribonucleotide reductase (RNR). During the last few decades, TrxR-like ferredoxin/flavodoxin NADP oxidoreductases (FNRs) have been discovered and characterized in several types of bacteria, including those not encoding the canonical plant-type FNR. In Bacillus cereus, a TrxR-like FNR has been shown to reduce the flavodoxin-like protein NrdI in the activation of class Ib RNR. However, some species only encode TrxR and lack the homologous TrxR-like FNR. Due to the struc... More