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Mutant Libraries> | … For other VHHs and their mutants, the genes encoding WT VHHs (D2-L19, cAb-Lys3 and D2-L24) were synthesized by Genscript based on the amino-acidic sequence (7) and optimized for expression in Escherichia coli. All the genes were cloned into the expression vector … | Get A Quote |
A substantial body of work has been carried out describing the structural features of the complex between single-domain antibodies (VHHs) and antigens, and the preeminence for epitopes located at concave surfaces of the antigen. However, the thermodynamic basis of binding is far less clear. Here, we have analyzed the energetic profiles of five VHHs binding to the catalytic cleft as well as a non-cleft epitope of hen egg lysozyme. Various binding energetic profiles with distinctive enthalpic/entropic contributions and structural distribution of critical residues were found in the five antibodies analyzed. Collectively, we suggest that from an energetic point of view the binding mechanism is influenced by the sha... More