Products/Services Used | Details | Operation |
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Mutagenesis Services> | … FLAG-TNK1 expression plasmid was purchased from GenScript. All mutagenesis was performed using the Q5 Site-Directed Mutagenesis Kit (NEB), following the manufacturer’s protocol. A complete list of all primers used is included in a table in Supplementary Information. … | Get A Quote |
TNK1 is a non-receptor tyrosine kinase with poorly understood biological function and regulation. Here, we identify TNK1 dependencies in primary human cancers. We also discover a MARK-mediated phosphorylation on TNK1 at S502 that promotes an interaction between TNK1 and 14-3-3, which sequesters TNK1 and inhibits its kinase activity. Conversely, the release of TNK1 from 14-3-3 allows TNK1 to cluster in ubiquitin-rich puncta and become active. Active TNK1 induces growth factor-independent proliferation of lymphoid cells in cell culture and mouse models. One unusual feature of TNK1 is a ubiquitin-association domain (UBA) on its C-terminus. Here, we characterize the TNK1 UBA, which has high affinity for poly-ubiqui... More