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Monoclonal Antibody Services> | … The cells were then treated with 4 μM Dox to express Cas9 protein and electrotransfected with synthesized sgRNA (GenScript). The single cell sorting was performed on a Beckman MoFlo Astrios EQs instrument (Beckman), and the developed monoclonal populations were … | Get A Quote |
Initiation is the rate-limiting step in translation, and its dysregulation is vital for carcinogenesis, including hematopoietic malignancy. Thus, discovery of novel translation initiation regulators may provide promising therapeutic targets. Here, combining Ribo-seq, mass spectrometry, and RNA-seq datasets, we discovered an oncomicropeptide, APPLE (a peptide located in ER), encoded by a non-coding RNA transcript in acute myeloid leukemia (AML). APPLE is overexpressed in various subtypes of AML and confers a poor prognosis. The micropeptide is enriched in ribosomes and regulates the initiation step to enhance translation and to maintain high rates of oncoprotein synthesis. Mechanically, APPLE promotes PABPC1-eIF... More