至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation

Mol Cell. 2021-09; 
Linyu Sun, Wentao Wang, Cai Han, Wei Huang, Yumeng Sun, Ke Fang, Zhancheng Zeng, Qianqian Yang, Qi Pan, Tianqi Chen, Xuequn Luo, Yueqin Chen
Products/Services Used Details Operation
Monoclonal Antibody Services … The cells were then treated with 4 μM Dox to express Cas9 protein and electrotransfected with synthesized sgRNA (GenScript). The single cell sorting was performed on a Beckman MoFlo Astrios EQs instrument (Beckman), and the developed monoclonal populations were … Get A Quote

摘要

Initiation is the rate-limiting step in translation, and its dysregulation is vital for carcinogenesis, including hematopoietic malignancy. Thus, discovery of novel translation initiation regulators may provide promising therapeutic targets. Here, combining Ribo-seq, mass spectrometry, and RNA-seq datasets, we discovered an oncomicropeptide, APPLE (a peptide located in ER), encoded by a non-coding RNA transcript in acute myeloid leukemia (AML). APPLE is overexpressed in various subtypes of AML and confers a poor prognosis. The micropeptide is enriched in ribosomes and regulates the initiation step to enhance translation and to maintain high rates of oncoprotein synthesis. Mechanically, APPLE promotes PABPC1-eIF... More

关键词

Micropeptide, PABPC1, cancer development, eIF4F complex assembly, eIF4G, leukemia, mRNA circularization, non-coding RNA (ncRNA), small open reading frame (ORF), translation initiation