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YAP facilitates melanoma migration through regulation of actin-related protein 2/3 complex subunit 5 (ARPC5)

Pigment Cell Melanoma Res. 2021-09; 
Jason W Lui, Stephen P G Moore, Lee Huang, Kelsey Ogomori, Yan Li, Deborah Lang
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Molecular Biology Tools … axis utilizing a photoshop measurement tool (PS Version CS6, Adobe Inc). 200 cells were counted per experiment, performed in triplicate. … Constructs that expressed high levels of ARPC5 from a CMV promoter were obtained from GenScript USA Inc. (ARPC5_pcDNA3.1(+), cat. … Get A Quote

摘要

Yes-associated protein 1 (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) are transcriptional coactivators that have been implicated in driving metastasis and progression in many cancers, mainly through their transcriptional regulation of downstream targets. Although YAP and TAZ have shown redundancy in many contexts, it is still unknown whether or not this is true in melanoma. Here, we show that while both YAP and TAZ are expressed in a panel of melanoma cell lines, depletion of YAP results in decreased cell numbers, focal adhesions, and the ability to invade matrigel. Using non-biased RNA-sequencing analysis, we find that melanoma cells depleted of YAP, TAZ, or YAP/TAZ exhibit drastically di... More

关键词

ARP2/3 complex, cancer, melanoma, transcriptional coactivator, yes-associated protein (YAP)