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Critical Conditions for Studying Interleukin-11 Signaling In Vitro and Avoiding Experimental Artefacts

Curr Protoc. 2021-09; 
Sivakumar Viswanathan, Benjamin Ng, Anissa A Widjaja, Chee Jian Pua, Nevin Tham, Jessie Tan, Stuart A Cook, Sebastian Schafer
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Mammalian Expression … Data in this article was generated using recombinant human IL11 protein purchased from Genscript. We have also confirmed that recombinant IL11 from another commercial source (Thermo Fisher Scientific) is equally effective in inducing human myofibroblast activation. … Get A Quote

摘要

Interleukin (IL) 11 is a member of the IL6 family of cytokines which require the ubiquitous gp130 receptor to activate canonical (JAK/STAT) and non-canonical (e.g., ERK) signaling pathways. The IL11 cytokine is upregulated in a number of fibro-inflammatory diseases and cancer, where it binds the cognate IL11 receptor alpha subunit (IL11RA) to form a hexameric IL11:IL11RA:gp130 signaling complex. The specific IL11RA receptor is highly expressed on cells of the stromal and parenchymal niche but expressed at low levels on immune cells, highly passaged cells, or transformed cell lines. Consequently, primary cells such as hepatic stellate cells, fibroblasts, and hepatocytes are ideal experimental systems to study IL... More

关键词

Interleukin 11, fibroblasts, fibrosis, hepatic stellate cells, hepatocytes, primary cell culture