至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Two evolutionarily duplicated domains individually and post-transcriptionally control SWEET expression for phloem transport

New Phytol. 2021-09; 
Chen Zhang, Ye Li, Jiang Wang, Xueyi Xue, Gabriel Beuchat, Li-Qing Chen
Products/Services Used Details Operation
GenParts™ DNA Fragments … The 3 biotinylated RNA fragment of TM7 and TM7∆33 was synthesized by GenScript. Potential RNA binding proteins were sought using Pierce™ Magnetic RNA-Protein Pull-Down Kit (Thermo Scientific) according to the manufacturer’s protocol with slight modifications. In brief, … Get A Quote

摘要

Cell type-specific gene expression is critical for the specialized functions within multicellular organisms. In Arabidopsis, SWEET11 and SWEET12 sugar transporters are specifically expressed in phloem parenchyma (PP) cells and are responsible for sucrose efflux from the PP, the first step of a two-step apoplasmic phloem-loading strategy that initiates the long-distance transport of sugar from leaves to nonphotosynthetic sink tissues. However, we know nothing about what determines the PP cell-specific expression of these SWEETs. Sequence deletions, histochemical β-glucuronidase (GUS) analysis, cross-sectioning, live-cell imaging, and evolutionary analysis were used to elucidate domains responsible for PP s... More

关键词

SWEET transporter, coevolution, phloem parenchyma, post-transcriptional regulation, specific expression, vascular plants