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DNA Sequencing> | … Primers for qPCR were designed with GenScript (www.genscript.com/tools/pcr-primers-designer). Target specificities of the primers were confirmed by sequencing the amplification product. The gene encoding ribosomal subunit L21 was used as a reference gene (Lehtonen et … | Get A Quote |
Plant viruses are important pathogens able to overcome plant defense mechanisms using their viral suppressors of RNA silencing (VSR). Small RNA pathways of bryophytes and vascular plants have significant similarities, but little is known about how viruses interact with mosses. This study elucidated the responses of Physcomitrella patens to two different VSRs. We transformed P. patens plants to express VSR P19 from tomato bushy stunt virus and VSR 2b from cucumber mosaic virus, respectively. RNA sequencing and quantitative PCR were used to detect the effects of VSRs on gene expression. Small RNA (sRNA) sequencing was used to estimate the influences of VSRs on the sRNA pool of P. patens. Expression of either VSR-... More