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DNA Sequencing> | … The PCR product was Sanger sequenced at GenScript and the 199 sequences were checked and trimmed for low quality bases in the software FinchTV 200 (http://www.geospiza.com/finchtv/). To identify the fungi, we used the BLAST tool in the NCBI 201 database. Sequences … | Get A Quote |
Geothermal soils offer unique insight into the way extreme environmental factors shape communities of organisms. However, little is known about the fungi growing in these environments and in particular how localized steep abiotic gradients affect fungal diversity. We used metabarcoding to characterize soil fungi surrounding a hot spring-fed thermal creek with water up to 84 °C and pH 10 in Yellowstone National Park. We found a significant association between fungal communities and soil variable principal components, and we identify the key trends in co-varying soil variables that explain the variation in fungal community. Saprotrophic and ectomycorrhizal fungi community profiles followed, and were significant... More