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DNA Sequencing> | … The N-terminal domain of ESCIT (Ser49-Ser269, which excludes the 48- GenScript (Piscataway, USA) residue mitochondrial targeting sequence and is hereafter referred to as N-ECSIT), and the C-terminal domain of ESCIT (Leu249-Ser431, referred to as C-ECSIT), were also … | Get A Quote |
The dual function protein ACAD9 catalyzes α,β-dehydrogenation of fatty acyl-CoA thioesters in fatty acid β-oxidation and is an essential chaperone for mitochondrial respiratory complex I (CI) assembly. ACAD9, ECSIT, and NDUFAF1 interact to form the core mitochondrial CI assembly complex. Current studies examine the molecular mechanism of ACAD9/ECSIT/NDUFAF1interactions. ACAD9 binds to the carboxy-terminal half and NDUFAF1 to the amino-terminal half of ECSIT. Binary complexes are unstable and aggregate easily, while the ACAD9/ECSIT/NDUFAF1 ternary complex is soluble and highly stable. Molecular modeling and small-angle X-ray scattering studies identified intra-complex interaction sites and binding sites for o... More