至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Oriented in situ immobilization of a functional tyrosinase on microcrystalline cellulose effectively incorporates DOPA residues in bioengineered mussel adhesive protein

Biotechnol J. 2021-09; 
Suhyeok Kim, Gaeun Bae, Mincheol Shin, Eungsu Kang, Tae Yoon Park, Yoo Seong Choi, Hyung Joon Cha
Products/Services Used Details Operation
DNA Sequencing … The gene sequence of the linker and CBM was codon-optimized, and chemically synthesized (GenScript, USA), and introduced into the pET22b+ vector (Novagen, Germany) using HindIII and XhoI. Then, the mTyr-CNK gene was obtained from the pmTyr-CNK vector[10a] and … Get A Quote

摘要

background: Catechol-containing polymers such as mussel adhesive proteins (MAPs) are attractive as biocompatible adhesive biomaterials, and the catecholic amino acid 3,4-dihydroxyphenyl-L-alanine (DOPA) is considered a key molecule in underwater mussel adhesion. Tyrosinases can specifically convert tyrosine to DOPA without any cofactors. However, their catalytic properties still need to be adjusted to minimize unwanted DOPA oxidation via their diphenolase activity and catechol instability at neutral and basic pH values in the reaction products. results: In this work, we constructed a novel functional tyrosinase, mTyr-CNK_CBM, by fusion of mTyr-CNK with a cellulose-binding motif (CBM) for oriented in situ immobi... More

关键词

3,4-Dihydroxyphenyl-L-alanine (DOPA), cellulose-binding motif, immobilization, mussel adhesive protein, tyrosinase