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DNA Sequencing> | … The ITS sequence of the pathogen used for species-specific primer design (using GenScript R ; GenScript, Piscataway, New Jersey, USA) had an amplicon size of approximately 150 bp. Fungal genomic DNA from the field-isolated strain was used to quantify the inoculum using … | Get A Quote |
unassigned: A comprehensive field-based screening protocol is lacking for dry root rot (DRR) disease in chickpea, which is caused by (formerly referred to as ). Here, we describe a protocol for establishing a sick plot for DRR to enable disease assessment of a large number of chickpea plants during the natural growing season. unassigned: We used a chickpea plot with >30% DRR incidence, and enriched the inoculum by cultivating highly susceptible chickpea plant genotypes and incorporating infected plant material into the soil. The chickpea plants were then subjected to infection in developed sick plots with various levels of soil moisture under natural field conditions. unassigned: Our protocol provides a robust... More