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Location and expression kinetics of Tc24 in different life stages of Trypanosoma cruzi

PLoS Negl Trop Dis. 2021-09; 
Leroy Versteeg, Rakesh Adhikari, Cristina Poveda, Maria Jose Villar-Mondragon, Kathryn M Jones, Peter J Hotez, Maria Elena Bottazzi, Edwin Tijhaar, Jeroen Pollet
Products/Services Used Details Operation
DNA Sequencing … Four different peptides were purchased (GenScript Biotech) which included 1) TAEAKQRR: the expected epitope, 2) PREKTAEAKQRRIEL: the expected epitope with the flanking peptide sequences used in the linear epitope prediction assay (PEPperPRINT), 3) … Get A Quote

摘要

Tc24-C4, a modified recombinant flagellar calcium-binding protein of Trypanosoma cruzi, is under development as a therapeutic subunit vaccine candidate to prevent or delay progression of chronic Chagasic cardiomyopathy. When combined with Toll-like receptor agonists, Tc24-C4 immunization reduces parasitemia, parasites in cardiac tissue, and cardiac fibrosis and inflammation in animal models. To support further research on the vaccine candidate and its mechanism of action, murine monoclonal antibodies (mAbs) against Tc24-C4 were generated. Here, we report new findings made with mAb Tc24-C4/884 that detects Tc24-WT and Tc24-C4, as well as native Tc24 in T. cruzi on ELISA, western blots, and different imaging tech... More

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