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DNA Sequencing> | … The coding sequence of codon-optimized human BRPF3 bromodomain region (residues 590-703) was synthesized and cloned (Genscript) into the pET28a(+)-TEV expression vector using NdeI and XhoI restriction sites. The pET28a(+)-TEV containing BRPF3 bromodomain … | Get A Quote |
Bromodomains are evolutionarily conserved reader modules that recognize acetylated lysine residues on the histone tails to facilitate gene transcription. The bromodomain and PHD finger containing protein 3 (BRPF3) is a scaffolding protein that forms a tetrameric complex with HBO1 histone acetyltransferase (HAT) and two other subunits, which is known to regulate the HAT activity and substrate specificity. However, its molecular mechanism, histone ligands, and biological functions remain unknown. Herein, we identify mono- (H4K5ac) and di- (H4K5acK12ac) acetylated histone peptides as novel interacting partners of the BRPF3 bromodomain. Consistent with this, pull-down assays on purified histones from human cells co... More