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Fusion of cellobiose phosphorylase and potato alpha-glucan phosphorylase facilitates substrate channeling for enzymatic conversion of cellobiose to starch

Prep Biochem Biotechnol. 2021-09; 
Xinyu Liu, Huawei Hou, Yapeng Li, Sen Yang, Hui Lin, Hongge Chen
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DNA Sequencing … coli and synthesized by GenScript (Piscataway, NJ, USA). The codon-optimized pgp sequence was deposited in GenBank under the accession number MZ821655. In our previous work, we have constructed pET20b-ctcbp and pET20b-pgp plasmids. … Get A Quote

摘要

We previously reported an enzymatic pathway for conversion of nonfood cellulose to starch (,110 (18): 7182-7187, 2013), in which the two sequential enzymes cellobiose phosphorylase (CBP) from and potato alpha-glucan phosphorylase (PGP) from were the two key enzymes responsible for the whole conversion rate. In this work CBP and PGP were fused to form a large enzyme and it turned out that the fusion protein could exhibit a good bifunctionality when PGP moiety was put at the N-terminus and CBP moiety at the C-terminus (designated as PGP-CBP). Although the coupled reaction rate of PGP-CBP was decreased by 23.0% compared with the free enzymes, substrate channeling between the two active sites in PGP-CBP was form... More

关键词

Amylose, cellobiose phosphorylase, fusion protein, potato alpha-glucan phosphorylase, substrate channeling