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DNA Sequencing> | … While the acrAB and oqxAB, along with a constitutive promoter sequence, were fused with a vector backbone containing the pBR322_origin and Ampicillin resistance gene (from the vector pTrc99a, Genscript) using the NdeI and HindIII restriction sites. The strains used for the … | Get A Quote |
OqxB is an RND (Resistance-Nodulation-Division) efflux pump that has emerged as a factor contributing to the antibiotic resistance in Klebsiella pneumoniae. OqxB underwent horizontal gene transfer and is now seen in other Gram-negative bacterial pathogens including Escherichia coli, Enterobacter cloacae and Salmonella spp., further disseminating multi-drug resistance. In this study, we describe crystal structure of OqxB with n-dodecyl-β-D-maltoside (DDM) molecules bound in its substrate-binding pocket, at 1.85 Å resolution. We utilize this structure in computational studies to predict the key amino acids contributing to the efflux of fluoroquinolones by OqxB, distinct from analogous residues in related tran... More