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Custom Vector Construction> | … The has-circ_0003420 (pCD-ciR-circ_0003420) and IGF2BP1 overexpression vectors (pcDNA3.1-IGF2BP1) were provided by Genscript Co., Ltd. (Nanjing, China). We used the synthetic vectors according to the supplier’s instructions and Lipofectamine 3000 (Invitrogen, … | Get A Quote |
unassigned: To explore the effect of circular RNA-0003420 (circ_0003420) in acute myeloid leukemia (AML) relapse and leukemia stem cells (LSCs) properties. unassigned: TRIzol reagent was used to extract total RNA from AML tissues or cells. Cell viability was assessed by Cell Counting Kit-8 and EdU staining assays. Cell apoptosis was determined by flow cytometry. unassigned: Compared with normal hematopoietic stem cells, circular RNA hsa-circ_0003420 expression was considerably decreased in non-m3 AML stem cells. Furthermore, the lack of hsa-circ_0003420 is correlated with poor clinical results and impaired therapeutic effects in AML. Overexpression of hsa-circ_0003420 via transfection caused LSC death and inhib... More