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Propofol induces the ferroptosis of colorectal cancer cells by downregulating STAT3 expression

Oncol Lett. 2021-09; 
Xining Zhao, Fei Chen
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Custom Vector Construction … The human STAT3 cDNA sequence was synthesized by GenScript and inserted into a pcDNA3.1 vector (Invitrogen; Thermo Fisher Scientific, Inc.). SW480 cells (70–90% confluence) were seeded into 6-well plates and transfected with STAT3 gene recombinant vector pcDNA3.1… Get A Quote

摘要

Propofol is a commonly used intravenous anesthetic agent that can also suppress the proliferation of various human cancer types, including colorectal cancer (CRC). The present study aimed to investigate whether propofol could induce the ferroptosis of CRC cells by regulating signal transducer and activator of transcription 3 (STAT3). STAT3 expression in normal and CRC tissues was measured. Human normal colonic epithelial NCM460 cells and human CRC SW480 cells were exposed to different concentrations of propofol and then cell viability was detected. SW480 cells were transfected with a vector overexpressing STAT3 and treated with propofol, and the cell viability, colony formation, cell proliferation, iron level, ... More

关键词

colorectal cancer, ferroptosis, propofol, signal transducer and activator of transcription 3