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Custom Vector Construction> | … Full-length tonB and its native promoter were cloned into pUC57 (Genscript) and then subcloned into pGEN_MCS 27 at BamHI and EcoRI sites to generate pGEN_tonB (Supplementary Table S1). Clones were verified by PCR with primers that bind to the vector (Supplementary … | Get A Quote |
Copper (Cu) is a key transition metal that is involved in many important biological processes in a cell. Cu is also utilized by the immune system to hamper pathogen growth during infection. However, genome-level knowledge on the mechanisms involved in adaptation to Cu stress is limited. Here, we report the results of a genome-wide reverse genetic screen for Cu-responsive phenotypes in Escherichia coli. Our screen has identified novel genes involved in adaptation to Cu stress in E. coli. We detected multiple genes involved in the biosynthesis and uptake of enterobactin, a siderophore utilized for high-affinity TonB-dependent acquisition of iron (Fe), as critical players in survival under Cu intoxication. We demo... More